Isotope Labeling-Based Quantitative Proteomics of Developing Seeds of Castor Oil Seed (Ricinus communisL.)
نویسندگان
چکیده
منابع مشابه
Differential Expression Patterns of Lipid Genes in Developing Seeds of Castor Bean (ricinus Communis L.)
Castor bean (Ricinus communis L.) is one of the most important non-edible oilseed crops. Characterization of seed development and lipid accumulation from female flowers pollinated to mature seeds has been carried out. The temporal expression patterns of 65 lipid genes involved in fatty acid and triacylglycerol biosynthesis using the semi-quantitative RT-PCR technique were also investigated. The...
متن کاملExtraction and Characterization of Castor Seed Oil from Wild Ricinus Communis Linn
Castor oil was extracted by mechanical cold pressing (< 45 0 C) of the seeds of wild castor plant (Ricinus communis L.) found in wet marginal lands of Katsina, Nigeria. Oil yield was 39.43%. The virgin oil (obtained as clear, viscous, pale-yellow liquid) was further purified by filtration through sintered glass (fine mesh screen). This was to eliminate any dust, gum, or other particulates prese...
متن کاملOStable Isotope Labeling in MS-based Proteomics
A variety of stable isotope labeling techniques have been developed and used in mass spectrometry (MS)-based proteomics, primarily for relative quantitation of changes in protein abundances between two compared samples, but also for qualitative characterization of differentially labeled proteomes. Differential O/O coding relies on the O exchange that takes place at the C-terminal carboxyl group...
متن کاملStudies on Ricinus Lipase Enzyme Isolated from Castor Seeds
Healthy and bold seeds of castor variety “Aruna” were collected. The de-hulled castor kernels were separated from seed coat and finely grounded in an electric grinder. The powdered kernels were passed through 60 mesh sieve and defatted using n-hexane in a soxhlet extractor. After complete extraction of oil seed meal was made free from solvent at low temperature. The defatted seed meal was pulve...
متن کاملStable Isotope Labeling in Proteomics
Proteomics, the analysis of the proteins expressed by a cell, tissue or organism under a specific set of conditions, has undergone a tremendous period of growth in the past few years. Proteomic studies are typically designed to analyze hundreds or thousands of proteins in a single analysis and aim to provide a global view of changes in protein expression that occur in different cellular growth ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Proteome Research
سال: 2013
ISSN: 1535-3893,1535-3907
DOI: 10.1021/pr400685z